Ayesha Takia was born on April 10, 1986 in Mumbai and grew up there. Her father Nishit is a Gujarati and mother Faridah, an anglo-Indian Muslim, she is the elder among two daughters. Ayesha attended St. Anthony's High School but never went to college because she entered the world of entertainment at very young age. Ayesha grew up speaking English at home and she worked hard to learn Hindi and Telugu for her roles in movies.

Ayesha's favorite actors from childhood are Aamir Khan and Shahrukh Khan. Her favorite actresses are Sridevi and Rani Mukherji. She finds that there are many good directors but she has always liked Sanjay Leela Bhansali.

She is a vegetarian, and enjoys going for long drives, reading, spending time with family, amongst others.

Ayesha Takia would like to be remembered as the cleanest actress in Bollywood. She has reportedly refusing roles that necessitates her to wear revealing and skimpy clothes and even refusing to do intimate scenes with co-stars.

Speaking to the media, Ayesha Takia said, "There're things that I wouldn't do, like kiss and wear certain kinds of clothes. I don't mind losing out on roles. At least 10 years from now I don't want to look back and cringe at anything. I've a family and a boyfriend. And I don't want to embarrass anyone." Ayesha further added, "I'm very happy with the work that I'm getting and I'm being taken seriously as an actress," said the self-styled virtuous actress.